vector blue Search Results


h 3501  (Vector Laboratories)
95
Vector Laboratories h 3501
H 3501, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h 3501/product/Vector Laboratories
Average 95 stars, based on 1 article reviews
h 3501 - by Bioz Stars, 2026-05
95/100 stars
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blue alkaline phosphatase substrate kit  (Vector Laboratories)
96
Vector Laboratories blue alkaline phosphatase substrate kit
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Blue Alkaline Phosphatase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blue alkaline phosphatase substrate kit/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
blue alkaline phosphatase substrate kit - by Bioz Stars, 2026-05
96/100 stars
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pst blue-1 vector  (Promega)
90
Promega pst blue-1 vector
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Pst Blue 1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pst blue-1 vector/product/Promega
Average 90 stars, based on 1 article reviews
pst blue-1 vector - by Bioz Stars, 2026-05
90/100 stars
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pt7 blue vector  (Merck & Co)
90
Merck & Co pt7 blue vector
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Pt7 Blue Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pt7 blue vector/product/Merck & Co
Average 90 stars, based on 1 article reviews
pt7 blue vector - by Bioz Stars, 2026-05
90/100 stars
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ptt5 expression vector  (Blue Heron Biotech)
90
Blue Heron Biotech ptt5 expression vector
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Ptt5 Expression Vector, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptt5 expression vector/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
ptt5 expression vector - by Bioz Stars, 2026-05
90/100 stars
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plasmid expression vectors encoding intact ul128, ul130, or ul131 proteins (strain towne)  (Blue Heron Biotech)
90
Blue Heron Biotech plasmid expression vectors encoding intact ul128, ul130, or ul131 proteins (strain towne)
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Plasmid Expression Vectors Encoding Intact Ul128, Ul130, Or Ul131 Proteins (Strain Towne), supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid expression vectors encoding intact ul128, ul130, or ul131 proteins (strain towne)/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
plasmid expression vectors encoding intact ul128, ul130, or ul131 proteins (strain towne) - by Bioz Stars, 2026-05
90/100 stars
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dna vaccine vector vr10551  (Blue Heron Biotech)
90
Blue Heron Biotech dna vaccine vector vr10551
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Dna Vaccine Vector Vr10551, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna vaccine vector vr10551/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
dna vaccine vector vr10551 - by Bioz Stars, 2026-05
90/100 stars
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pair of vectors expressing mutant toxins lacking cysteine residues  (Blue Heron Biotech)
90
Blue Heron Biotech pair of vectors expressing mutant toxins lacking cysteine residues
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Pair Of Vectors Expressing Mutant Toxins Lacking Cysteine Residues, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pair of vectors expressing mutant toxins lacking cysteine residues/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
pair of vectors expressing mutant toxins lacking cysteine residues - by Bioz Stars, 2026-05
90/100 stars
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lmp-blue fluorescent protein (bfp) vectors  (BIODEV Formation et Consulting)
90
BIODEV Formation et Consulting lmp-blue fluorescent protein (bfp) vectors
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Lmp Blue Fluorescent Protein (Bfp) Vectors, supplied by BIODEV Formation et Consulting, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lmp-blue fluorescent protein (bfp) vectors/product/BIODEV Formation et Consulting
Average 90 stars, based on 1 article reviews
lmp-blue fluorescent protein (bfp) vectors - by Bioz Stars, 2026-05
90/100 stars
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pt7-guide vector  (Blue Heron Biotech)
90
Blue Heron Biotech pt7-guide vector
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Pt7 Guide Vector, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pt7-guide vector/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
pt7-guide vector - by Bioz Stars, 2026-05
90/100 stars
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pucminusmcs vector shuttle  (Blue Heron Biotech)
90
Blue Heron Biotech pucminusmcs vector shuttle
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Pucminusmcs Vector Shuttle, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pucminusmcs vector shuttle/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
pucminusmcs vector shuttle - by Bioz Stars, 2026-05
90/100 stars
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pmos-blue vector  (Promega)
90
Promega pmos-blue vector
Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
Pmos Blue Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmos-blue vector/product/Promega
Average 90 stars, based on 1 article reviews
pmos-blue vector - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

Techniques: Cloning, Staining, Cell Culture

Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

Techniques: Amplification, Sequencing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus

Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

Techniques: Amplification, Sequencing, Mutagenesis, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus